重組人粒細(xì)胞刺激因子

Granulocyte colony-stimulating factor (G-CSF) 是一種由免疫系統(tǒng)細(xì)胞，成纖維細(xì)胞和內(nèi)皮細(xì)胞分泌的糖蛋白，是造血和內(nèi)皮前體細(xì)胞的細(xì)胞因子。G-CSF 刺激骨髓中成熟的祖細(xì)胞分化為粒細(xì)胞，巨噬細(xì)胞和 T 細(xì)胞。G-CSF 還能夠通過增加 PI3K/Akt 通路的磷酸化為中樞神經(jīng)元提供神經(jīng)保護(hù)，并調(diào)節(jié)癌癥中的上皮到間質(zhì)轉(zhuǎn)化。

(純度) Purity

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).

(內(nèi)毒素) Endotoxin

≤10 EU/mg by the LAL method.

(生物活性) Bioactivity

The bioactivity is determined by the dose-dependent proliferation of murine NFS-60 cells is ＜ 0.5 ng/mL, corresponding to a specificBioactivity of ≥ 1 x 107 units/mg.

(種屬) Species

Human

(表達(dá)系統(tǒng)) Expression System

CHO

(預(yù)測分子量大小) Mol. Weight

18.7 kDa

(表達(dá)區(qū)間) Expression Region

Thr31-Pro204

(標(biāo)簽) Tag Info

Tag free

(劑型) Formulation

Lyophilized

(溶液) Buffer

PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.

(規(guī)格) Size

10μg/50μg/1mg

(復(fù)溶) Reconstitution

It is recommended to redissolve in sterile deionized water.

(運輸) Shipping

The product is shipped with blue ice.

(存儲) Storage & Stability

36 months at -20°C to -80°C in lyophilized state;

6 months at -20°C to -80°C under sterile conditions after reconstitution;

7-10 days at 2°C to 8°C under sterile conditions after reconstitution;

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.?

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